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ISSN Druckformat: 0731-8898
ISSN Online: 2162-6537
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Evaluation of Potential of Long Noncoding RNA NEAT1 in Colorectal Cancer
ABSTRAKT
Long noncoding RNAs (lncRNAs) have been reported to be involved in cancer initiation and evolution, including colorectal cancer (CRC). Nuclear-enriched abundant transcript 1 (NEAT1) exerts important functions in multiple cancers; however, the specific modulatory mechanism in CRC demands in-depth exploration. The expression levels of NEAT1, microRNA-195-5p (miR-195-5p), and centrosomal protein 55 (CEP55) were examined using quantitative real-time polymerase chain reaction (qRT-PCR), and protein expression of CEP55 was detected by Western blot assay. Cell proliferation and apoptosis were measured by 3-(4,5-dimethylthiazole-2-y1)-2,5-diphenyl tetrazolium bromide (MTT) assay and flow cytometry. Transwell migration and invasion assays were applied to evaluate cell metastasis ability. Dual-luciferase reporter assay was used to analyze the correlation among NEAT1, miR-195-5p and CEP55. The expression of NEAT1 was up-regulated in CRC tissues and cells, and overall survival was lower with high expression of NEAT1. Knockdown of NEAT1 repressed cell proliferation, migration, and invasion, while inducing apoptosis in CRC cells. NEAT1 targeted miR-195-5p and inhibited the expression of miR-195-5p. Silence of NEAT1 inhibited CRC cell proliferation, migration, and invasion, and promoted apoptosis by up-regulating miR-195-5p. MiR-195-5p targeted and suppressed CEP55 expression, and CEP55 reverted the effects induced by miR-195-5p. NEAT1 regulated the expression of CEP55 through miR-195-5p. NEAT1 promotes colorectal cancer cellular processes by regulating CEP55 expression via the sponging of miR-195-5p. Therefore, NEAT1 might play a crucial role in CRC treatments.
-
Jemal A, Bray F, Center MM. Global cancer statistics. CA Cancer J Clin. 2011;61:69.
-
Brenner H, Kloor M, Pox CP. Colorectal cancer. Lancet. 2014;383:1490.
-
Walther A, Johnstone E, Swanton C, Midgley R, Tomlinson I, Kerr D. Genetic prognostic and predictive markers in colorectal cancer. Nature Rev Cancer. 2009 Jul;9(7):489-99.
-
Ponting CP, Oliver PL, Wolf R. Evolution and functions of long noncoding RNAs. Cell. 2009;136(4):629-41.
-
Nagano T, Fraser P. No-nonsense functions for long noncoding RNAs. Cell. 2011;145:178.
-
Chai Y, Liu J, Zhang Z. HuR-regulated lncRNA NEAT1 stability in tumorigenesis and progression of ovarian cancer. Cancer Med. 2016;5:1588.
-
Chakravarty D, Sboner A, Nair SS. The oestrogen receptor alpha-regulated lncRNA NEAT1 is a critical modulator of prostate cancer. Nat Commun. 2014;5:5383.
-
Qian K, Liu G, Tang Z. The long non-coding RNA NEAT1 interacting with miR-101 modulates breast cancer growth by targeting EZH2. Arch Biochem Biophys. 2017;615:1.
-
Zhang J, Zhao B, Chen X. Silence of long noncoding RNA NEAT1 inhibits malignant biological behaviors and chemotherapy resistance in gastric cancer. Pathol Oncol Res. 2018;24(1):109-13.
-
Cho WC. OncomiRs: The discovery and progress of microRNAs in cancers. Mol Cancer. 2007;6(1):60.
-
Gregory RI, Shiekhattar R. MicroRNA biogenesis and cancer. Cancer Res. 2011;65:3509.
-
Yang C, Wu K, Wang S. Long non-coding RNA XIST promotes osteosarcoma progression by targeting YAP via miR-195-5p. J Cell Biochem. 2018;119:5646.
-
Yang R, Xing L, Zheng X. The circRNA circAGFG1 acts as a sponge of miR-195-5p to promote triple-negative breast cancer progression through regulating CCNE1 expression. Mol Cancer. 2019;18:4.
-
Chai L, Kang XJ, Sun ZZ. MiR-497-5p, miR-195-5p and miR-455-3p function as tumor suppressors by targeting hTERT in melanoma A375 cells. Cancer Manag Res. 2018;10:989.
-
Jeffery J, Sinha D, Srihari S. Beyond cytokinesis: The emerging roles of CEP55 in tumorigenesis. Oncogene. 2016;35:683.
-
Jiang W, Wang Z, Jia Y. CEP55 overexpression predicts poor prognosis in patients with locally advanced esophageal squamous cell carcinoma. Oncol Lett. 2017;13:236.
-
Hauptman N, Jevsinek Skok D, Spasovska E. Genes CEP55, FOXD3, FOXF2, GNAO1, GRIA4, and KCNA5 as potential diagnostic biomarkers in colorectal cancer. BMC Med Genom. 2019;12:54.
-
Jen J, Tang YA, Lu YH. Oct4 transcriptionally regulates the expression of long non-coding RNAs NEAT1 and MALAT1 to promote lung cancer progression. Mol Cancer. 2017;16:104.
-
Zhou K, Zhang C, Yao H. Knockdown of long non-coding RNA NEAT1 inhibits glioma cell migration and invasion via modulation of SOX2 targeted by miR-132. Mol Cancer. 2018;17:105.
-
Wu Y, Yang L, Zhao J. Nuclear-enriched abundant tran-script 1 as a diagnostic and prognostic biomarker in colorectal cancer. Mol Cancer. 2015;14:191.
-
Peng W, Wang Z, Fan H. LncRNA NEAT1 impacts cell proliferation and apoptosis of colorectal cancer via regulation of Akt signaling. Pathol Oncol Res. 2017 Jul 1;23(3):651-6.
-
Yu HM, Wang C, Yuan Z, Chen GL, Ye T, Yang BW. Ln-cRNA NEAT1 promotes the tumorigenesis of colorectal cancer by sponging miR-193a-3p. Cell Proliferation. 2019 Jan;52(1):e12526.
-
Li JH, Zhang SQ, Qiu XG. Long non-coding RNA NEAT1 promotes malignant progression of thyroid carcinoma by regulating miRNA-214. Int J Oncol. 2017;50:708.
-
Xin T, Zhang G, Hui Z. Long non-coding RNA NEAT1 contributes to docetaxel resistance of prostate cancer through inducing RET expression by sponging miR-34a. Rsc Adv. 2017;7:42986.
-
Yan J. Long noncoding RNA NEAT1 mediates gastric carcinogenesis and cisplatin resistance by functioning as a competing endogenous RNA. Gastroenterology. 2017;152:S804.
-
Lin X, Wang S, Sun M, Zhang C, Wei C, Yang C, Dou R, Liu Q, Xiong B. miR-195-5p/NOTCH2-mediated EMT modulates IL-4 secretion in colorectal cancer to affect M2-like TAM polarization. J Hematol Oncol. 2019;12:20.
-
Wang T, Ren Y, Liu R. miR-195-5p suppresses the proliferation, migration, and invasion of oral squamous cell carcinoma by targeting TRIM14. Biomed Res Int. 2017;2017:7378148.
-
Qu Q, Chu X, Wang P. MicroRNA-195-5p suppresses osteosarcoma cell proliferation and invasion by suppressing naked cuticle homolog 1. Cell Biol Int. 2017;41:287.
-
Luo Q, Zhang Z, Dai Z. Tumor-suppressive microRNA-195-5p regulates cell growth and inhibits cell cycle by targeting cyclin dependent kinase 8 in colon cancer. Am J Transl Res. 2016;8:2088.
-
Lu Y, Li T, Wei G. The long non-coding RNA NEAT1 reg-ulates epithelial to mesenchymal transition and radiore-sistance through miR-204/ZEB1 axis in nasopharyngeal carcinoma. Tumour Biol. 2016;37:11733.
-
Zhen L, Yun-Hui L, Hong-Yu D. Long noncoding RNA NEAT1 promotes glioma pathogenesis by regulating miR-449b-5p/c-Met axis. Tumour Biol. 2016;37:673.
-
Luo Y, Chen JJ, Lv QL. Long non-coding RNA NEAT1 promotes colorectal cancer progression by competitively binding miR-34a with SIRT1 and enhancing the Wnt/beta-catenin signaling pathway. Cancer Lett. 2019;440-441:11.
-
Luo Jing, Xie Kai, Gao Xiang, Yao Yu, Wang Gaoming, Shao Chenye, Li Xiaokun, Xu Yang, Ren Binhui, Hu Liwen, Shen Yi, Long Noncoding RNA Nuclear Paraspeckle Assembly Transcript 1 Promotes Progression and Angiogenesis of Esophageal Squamous Cell Carcinoma Through miR-590-3p/MDM2 Axis, Frontiers in Oncology, 10, 2021. Crossref
-
Bai Xiang-feng, Niu Rui-ze, Liu Jia, Pan Xu-dong, Wang Feng, Yang Wei, Wang Lu-qiao, Sun Li-zhong, Roles of noncoding RNAs in the initiation and progression of myocardial ischemia–reperfusion injury, Epigenomics, 13, 9, 2021. Crossref
-
Zhou Yongfang, Zhang Fei, Xu Fengyang, Wang Qiang, Wu Jianhua, Peng Wuxun, Dong Wentao, lncRNA NEAT1 regulates CYP1A2 and influences steroid-induced necrosis, Open Life Sciences, 16, 1, 2021. Crossref
-
Chen Juan, Xu Li, Fang Mingzhi, Xue Yahong, Cheng Yan, Tang Xiuhong, Hsa_circ_0060927 participates in the regulation of Caudatin on colorectal cancer malignant progression by sponging miR‐421/miR‐195‐5p, Journal of Clinical Laboratory Analysis, 36, 5, 2022. Crossref
-
Xu Qi, Xu Jia-Lin, Chen Wen-Quan, Xu Wen-Xiu, Song Yu-Xin, Tang Wen-Juan, Xu Di, Jiang Meng-Ping, Tang Jinhai, Roles and mechanisms of miR-195–5p in human solid cancers, Biomedicine & Pharmacotherapy, 150, 2022. Crossref
-
Gu Jingtao, Zhang Bo, An Rui, Qian Weikun, Han Liang, Duan Wanxing, Wang Zheng, Ma Qingyong, Molecular Interactions of the Long Noncoding RNA NEAT1 in Cancer, Cancers, 14, 16, 2022. Crossref