Abo Bibliothek: Guest
Digitales Portal Digitale Bibliothek eBooks Zeitschriften Referenzen und Berichte Forschungssammlungen
Journal of Environmental Pathology, Toxicology and Oncology
Impact-faktor: 1.241 5-jähriger Impact-Faktor: 1.349 SJR: 0.356 SNIP: 0.613 CiteScore™: 1.61

ISSN Druckformat: 0731-8898
ISSN Online: 2162-6537

Journal of Environmental Pathology, Toxicology and Oncology

DOI: 10.1615/JEnvironPatholToxicolOncol.v20.i1.110
11 pages

Low Doses of Heavy Metals Disrupt Normal Structure and Function of Rat Platelets

Siragam Vinaya Kumar
Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University,West Bengal, India
Rakhi Bose
Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University,West Bengal, India
Shelley Bhattacharya
Environmental Toxicology Laboratory, Department of Zoology, Visva Bharati University,West Bengal, India

ABSTRAKT

Aggregation, an important property of platelets, plays a key role in the coagulation of blood and is potentiated by ADP and adrenaline, whereas cAMP acts as a strong inhibitor. Because of the rise in the heavy metal load in the environment, more studies are necessary to look at its subtle effects in the animal system, wherein platelet structure and function can be targeted. We carried out structural analyses under phase contrast and scanning electronmicroscopy of the platelets prepared from 1/10 LD50 metal-treated rats. After mercury (Hg) and arsenic (As) treatment, the cell margins appeared irregular and wavy, with small pseudopodia-like protrusions from the surface. Cadmium (Cd) treatment caused loss of the general spindle shape, and the platelets assumed a round spongy appearance. All metal treatments effected enhanced collagen-induced aggregation and inhibited ADP and arachidonic acid-induced aggregation, whereas epinephrine accelerated aggregation in Hg and Cd treatment but inhibited aggregation in As treatment. Cd proved a potent inhibitor of phosphodiesterase, increasing the cAMP level in in vitro treatments at equimolar (5, 10, and 20 pmoles) concentrations. The rate of aggregation was enhanced with all the agonists used in in vitro Hg and As treatments, with concomitant reduction in cAMP, while Cd inhibited platelet aggregation. Thus, we can conclude that cytosolic cAMP, which is decreased by the metal-induced inhibition of phosphodiesterase, is a regulator molecule in platelet aggregation. Furthermore, it is also abundantly clear that equimolar doses of metals are not always equitoxic. Therefore, the action of each xenobiotic is not only due to the concentration used but also to the type of xenobiotic depending on its unique mechanism of action.


Articles with similar content:

Antioxidative Effects of Schidandrin B and Green Tea Polyphenols Against Mercuric Chloride-Induced Hepatotoxicity in rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.33, 2014, issue 4
Tianyao Yang, Yu Deng, Shu Feng, Zhaofa Xu, Wei Liu, Bin Xu
Protective Role of Zinc in Ameliorating Arsenic-Induced Oxidative Stress and Histological Changes in Rat Liver
Journal of Environmental Pathology, Toxicology and Oncology, Vol.29, 2010, issue 2
Devinder K. Dhawan, Anshoo Malhotra, Ashok Kumar, Praveen Nair, M. L. Garg
The Cyclooxygenase-2 Inhibitor Etoricoxib Is a Potent Chemopreventive Agent of Colon Carcinogenesis in the Rat Model
Journal of Environmental Pathology, Toxicology and Oncology, Vol.28, 2009, issue 1
Sankar Nath Sanyal, Manpreet Kaur Saini, Pinky Sharma, Jasmeet Kaur
Characterization of Microcystin-Induced Dualistic Toxic Effects on Primary Rat Hepatocytes
Journal of Environmental Pathology, Toxicology and Oncology, Vol.36, 2017, issue 1
Xinyu Hong, Xiaolan Qian, Weijian Zhong, Jianxun Yin, Dan Lou, Yanqin Wang, Ping Xiao, Yi Shuai
Nuclear Factor Kappa B: A Pro-Inflammatory, Transcription Factor−Mediated Signalling Pathway in Lung Carcinogenesis and Its Inhibition By Nonsteroidal Anti-Inflammatory Drugs
Journal of Environmental Pathology, Toxicology and Oncology, Vol.31, 2012, issue 1
Sankar Nath Sanyal, Shruti Setia