RT Journal Article ID 5e7efbfd2e825d75 A1 Zhu, Lina A1 Tang, Qingjiu A1 Zhou, Shuai A1 Liu, Yanfang A1 Zhang, Zhong A1 Gao, Xinhua A1 Wang, Shiping A1 Wang, Zhaolong T1 Isolation and Purification of a Polysaccharide from the Caterpillar Medicinal Mushroom Cordyceps militaris (Ascomycetes) Fruit Bodies and Its Immunomodulation of RAW 264.7 Macrophages JF International Journal of Medicinal Mushrooms JO IJM YR 2014 FD 2014-06-04 VO 16 IS 3 SP 247 OP 257 K1 medicinal mushrooms K1 Cordyceps militaris K1 polysaccharide K1 monosaccharide composition K1 immunostimulating activity AB A novel polysaccharide (CP2-S) was purified from Cordyceps militaris fruit bodies by hot water extraction, ethanol precipitation, DEAE-Sepharose Fast Flow and Sephacryl S-400 high-resolution chromatography. The polysaccharide had a molecular weight of 5.938 × 106 g/mol and was mainly composed of glucose. CP2-S had carbohydrate content estimated to be 100% using the phenol-sulfuric acid method. Immunostimulating experiments in vitro indicated that CP2-S could stimulate nitric oxide production, phagocytosis, respiratory burst activity, and secretion of interleukin-1β and interleukin-2 of macrophages, suggesting that this water-soluble polysaccharide from the fruit body of C. militaris is a natural immunostimulating polysaccharide with potential for further application. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,23d62e9b62d52918,5e7efbfd2e825d75.html