%0 Journal Article %A Yin, Xiulian %A You, Qinghong %A Zhou, Xinghai %D 2015 %I Begell House %K edible and medicinal mushrooms, Agaricus bisporus polysaccharides, complex enzyme-assisted extraction, antioxidant activities %N 10 %P 987-996 %R 10.1615/IntJMedMushrooms.v17.i10.80 %T Complex Enzyme-Assisted Extraction, Purification, and Antioxidant Activity of Polysaccharides from the Button Mushroom, Agaricus bisporus (Higher Basidiomycetes) %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2fb21a9c4fd053ea,508a39314d762866.html %V 17 %X Agaricus bisporus polysaccharides (ABP) were extracted by complex enzyme-assisted extraction methodology. The following were optimal conditions for the extraction of crude ABP: complex enzyme amount, 2.2%; temperature, 62°C; time, 3 h; and pH, 4. Under these conditions, the experimental yield of crude ABP was 6.87%. The crude ABP was purified by diethylaminoethyl-cellulose 52 chromatography and Sephadex G-100 chromatography, and one fraction−namely, ABP-1−was produced. The ABP-1 contained 93.67% carbohydrate, 1.46% protein, and 0.62% uronic acid. The constituent monosaccharides were predominantly glucose, galactose, mannose, and xylose. The antioxidant activities of ABP-1 were investigated by measuring its scavenging ability on 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, its ferric-reducing activity power, and the reducing power assay. At a concentration of 1.2 mg/mL, ABP-1 seemed to possess good free radical scavenging activity, with a scavenging value of about 56%. The results indicate that ABP-1 has good antioxidant activity. %8 2016-01-07