RT Journal Article ID 43fc92dc1b096741 A1 Radic, Natasa A1 Jevnikar, Zala A1 Obermajer, Natasa A1 Kristl, Jernej A1 Kos, Janko A1 Pohleven, Franc A1 Strukelj, Borut T1 Infuence of Culinary-Medicinal Maitake Mushroom, Grifola frondosa (Dicks.: Fr.) S.F. Gray (Aphyllophoromycetideae) Polysaccharides on Gene Expression in Jurkat T Lymphocytes JF International Journal of Medicinal Mushrooms JO IJM YR 2010 FD 2010-08-24 VO 12 IS 3 SP 245 OP 255 K1 medicinal mushrooms K1 Maitake mushroom K1 Grifola frondosa K1 Jurkat T cells K1 differential display K1 gene expression AB The fruiting body of the culinary-medicinal Maitake mushroom Grifola frondosa contains β-glucans that have immunomodulatory effects and potential uses as adjuncts to cancer therapy. Numerous clinical trials are being conducted to assess the benefits of using commercial preparations containing G. frondosa extracts in cancer treatment. We tested a commercially available product containing polysaccharides from the G. frondosa (CGFP) and studied their effect on gene expression in Jurkat T cells. An mRNA differential display was performed to gain insight into the cell response to the substance. HAI1, hnRNP A2/B1, PKC iota, KLRC1, and genes of the ubiquitin system were identified as possible regulators in the mechanism of tumor-suppressor action of CGFP. Real-time polymerase chain reaction (qPCR) was used to investigate the effect of CGFP on cytokine expression in Jurkat T cells. The qPCR results showed that the expression of the gene for IL-5 is increased. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2da9c44d295f30f8,43fc92dc1b096741.html