RT Journal Article
ID 43fc92dc1b096741
A1 Radic, Natasa
A1 Jevnikar, Zala
A1 Obermajer, Natasa
A1 Kristl, Jernej
A1 Kos, Janko
A1 Pohleven, Franc
A1 Strukelj, Borut
T1 Infuence of Culinary-Medicinal Maitake Mushroom, Grifola frondosa (Dicks.: Fr.) S.F. Gray (Aphyllophoromycetideae) Polysaccharides on Gene Expression in Jurkat T Lymphocytes
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2010
FD 2010-08-24
VO 12
IS 3
SP 245
OP 255
K1 medicinal mushrooms
K1 Maitake mushroom
K1 Grifola frondosa
K1 Jurkat T cells
K1 differential display
K1 gene expression
AB The fruiting body of the culinary-medicinal Maitake mushroom Grifola frondosa contains β-glucans that have immunomodulatory effects and potential uses as adjuncts to cancer therapy. Numerous clinical trials are being conducted to assess the benefits of using commercial preparations containing G. frondosa extracts in cancer treatment. We tested a commercially available product containing polysaccharides from the G. frondosa (CGFP) and studied their effect on gene expression in Jurkat T cells. An mRNA differential display was performed to gain insight into the cell response to the substance. HAI1, hnRNP A2/B1, PKC iota, KLRC1, and genes of the ubiquitin system were identified as possible regulators in the mechanism of tumor-suppressor action of CGFP. Real-time polymerase chain reaction (qPCR) was used to investigate the effect of CGFP on cytokine expression in Jurkat T cells. The qPCR results showed that the expression of the gene for IL-5 is increased.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2da9c44d295f30f8,43fc92dc1b096741.html