RT Journal Article
ID 284327ed49df603c
A1 Grunewald, Franziska
A1 Steinborn, Carmen
A1 Huber, Roman
A1 Wille, Radim
A1 Meier, Stefanie
A1 Alresly, Zeyad
A1 Lindequist, Ulrike
A1 Gründemann, Carsten
T1 Effects of Birch Polypore Mushroom, Piptoporus betulinus (Agaricomycetes), the "Iceman’s Fungus", on Human Immune Cells
JF International Journal of Medicinal Mushrooms
JO IJM
YR 2018
FD 2018-11-30
VO 20
IS 12
SP 1135
OP 1147
K1 immunomodulation
K1 medicinal mushrooms
K1 Piptoporus betulinus
AB Piptoporus betulinus, the mushroom that has been carried by Ötzi the "Iceman", has a long tradition of use in medicinal practice for its antiseptic, anticancer, and immune-enhancing properties. With this study, we aimed to investigate the immunomodulatory effects of P. betulinus on primary human immunocompetent cells. The influence of P. betulinus water and methanol extract on apoptosis and necrosis induction of T cells and monocytes was analyzed using annexin V/propidium iodide staining and proliferation of T cells by carboxyfluorescein diacetate succinimidyl ester staining using flow cytometry. The effects on T-cell activation (CD69/CD25) and dendritic cell maturation (CD83, CD86, and CD14) were assessed using flow cytometric analysis of distinct marker expression. Alterations of the secretion of effector mediators interferon (IFN)-γ by T cells and interleukin (IL)-8 by monocytes and dendritic cells were detected by enzyme-linked immunosorbent assay. None of the P. betulinus extracts had a significant influence
on apoptosis and necrosis induction, T-cell proliferation, or T-cell activation status, but P. betulinus water extract caused a strong increase in IFN-γ release. The same extract was slightly protective against apoptosis of monocytes and further triggered IL-8 secretion by monocytes and dendritic cells. Moreover, P. betulinus water extract induced dendritic cell maturation. Our results demonstrate the immune-enhancing properties of P. betulinus.
PB Begell House
LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,2c6ab3993645ca9e,284327ed49df603c.html