%0 Journal Article %A Ishibashi, Ken-ichi %A Morita, Mayu %A Motoi, Masuro %A Liu, Ying %A Miura, Noriko N. %A Adachi, Yoshiyuki %A Ohno, Naohito %D 2013 %I Begell House %K medicinal mushrooms and fungi, β-glucan, anti-β-glucan antibody, class, reactivity %N 2 %P 115-126 %R 10.1615/IntJMedMushr.v15.i2.10 %T Analysis of the Titer and Reactivity of Antibody/ies Against Fungal Cell Wall β-Glucans in Human Sera %U https://www.dl.begellhouse.com/journals/708ae68d64b17c52,72e9ed69099c0eef,2048ae6d24e0c37e.html %V 15 %X The aim of this work was to characterize an antibody response to β-glucan (BG), a major component of the fungal cell wall, at each isotype in human sera. The titer and reactivity of the anti-human BG antibody were examined using enzyme-linked immunosorbant assay plates coated with Candida soluble cell wall β-glucan as a standard antigen. The antibody was detected using anti-human immunoglobulin (Ig) G, IgM, and IgA. Its major class was IgG in all subjects. The antibody titer varied significantly. The anti-BG antibody showed greater reactivity to β-glucans derived from pathogenic fungi than monoglycosyl-branched β-glucans derived from mycelia culture medium. In addition, it was suggested that the anti-BG IgM antibody was bound relatively strongly to the β1,3-glucan backbone and the anti-BG IgG antibody to β1,6-glucan. The anti-BG antibody plays a variety of roles, according to class, in the host's response to fungi. We propose a new index of human response to β-glucan that effects the understanding of the response to β-glucan in humans. %8 2013-03-22