RT Journal Article ID 1975433a6541caa9 A1 Zhang, Zong-Qi A1 Liu, Li-Ping A1 Lei, Lu A1 Wang, Chuan-Nan A1 Tang, Qing-Li A1 Wu, Tian-Xiang T1 Antioxidative and Immunomodulatory Activities of the Exopolysaccharides from Submerged Culture of Hen of the Woods or Maitake Culinary-Medicinal Mushroom, Grifola frondosa (Agaricomycetes) by Addition of Rhizoma gastrodiae Extract and Its Main Components JF International Journal of Medicinal Mushrooms JO IJM YR 2019 FD 2019-08-20 VO 21 IS 8 SP 825 OP 839 K1 Rhizoma gastrodiae extract K1 Grifola frondosa K1 antioxidant activity K1 RAW264.7 macrophages K1 p-hydroxylbenzaldehyde K1 immunomodulating activity K1 medicinal mushrooms AB Grifola frondosa (hen of the woods or maitake) is a famous culinary-medicinal mushroom, and its exopolysaccharides (EPSs) have biological activities with or without supplementation with exogenous additives. In this study, a Rhizoma gastrodiae extract was added to a G. frondosa fermentation system. P-hydroxylbenzaldehyde (HBA), the main product of R. gastrodiae, had the highest utilization rate in the fermentation process (42%). In addition, the EPSs of G. frondosa after addition of R. gastrodiae extract (REPS), of HBA (HEPS), or of a standard solution according to the main component ratio of R. gastrodiae extract (CEPS) were obtained. We then determined the antioxidant and immunomodulatory activities of EPS, REPS, HEPS, and CEPS. Overall, REPS showed the highest antioxidant activities compared with EPS and HEPS (P < 0.05) but similar to that of CEPS (P > 0.05). The half-inhibitory concentration (ED50) values of REPS (< 4 mg/mL) were lower than those of EPS, HEPS, and CEPS. Moreover, REPS was better able to stimulate phagocytosis and nitric oxide production of RAW 264.7 macrophages than were the others, without a significant difference from CEPS (P > 0.05). An interesting and important finding is that a R. gastrodiae extract can increase antioxidant and immunomodulatory activities of EPS preparations from G. frondosa, and the standard solution of the main components of the R. gastrodiae extract may be better for simulating fermentation performed by G. frondosa and biological activities of its major products. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,7004d4be6a1d3b42,1975433a6541caa9.html