RT Journal Article ID 029d31540328ce6b A1 Hernandez-Marquez, Eva A1 Lagunas-Martinez, Alfredo A1 Bermudez-Morales, Victor H. A1 Burgete-Garcfa, Ana I. A1 Leon-Rivera, Ismael A1 Montiel-Arcos, Elizur A1 Garcia-Villa, Enrique A1 Gariglio, Patricio A1 Madrid-Marina V., Vicente A1 Ondarza-Vidaurreta, Raul N. T1 Inhibitory Activity of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Higher Basidiomycetes) on Transformed Cells by Human Papillomavirus JF International Journal of Medicinal Mushrooms JO IJM YR 2014 FD 2014-04-22 VO 16 IS 2 SP 179 OP 187 K1 medicinal mushrooms K1 cancer cells K1 Ganoderma lucidum K1 human papillomavirus K1 apoptosis K1 cell cycle AB In this study, we investigated the effects of the aqueous extracts of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum, obtained from three localities (China; and Morelos and Michoacan, Mexico) on cervical cells transformed by human papillomavirus (HeLa and SiHa) and C-33A cancer cells. The cells were plated in DMEM medium supplemented, and were incubated in the presence of different concentrations of G. lucidum for 24 h. Cell proliferation was determined by MTT colorimetric assay and viability by trypan blue assay. Inhibitory dose was determined (IC50) of the three different extracts of G. lucidum in the culture cell lines mentioned above. The apoptosis process was confirmed by nuclear DNA fragmentation and the cell cycle was determined by flow cytometry. The results showed that aqueous extracts G. lucidum obtained from three localities produced inhibition in the proliferation of VPH transformed cells; they also induced apoptosis and cell cycle arrest in HeLa, SiHa, and C-33A cancer cells. Therefore, it was found that aqueous extracts G. lucidum obtained from three different locations produced inhibitory effect on cancer cells and may have a potential therapeutic use for the prevention and treatment of this disease. PB Begell House LK https://www.dl.begellhouse.com/journals/708ae68d64b17c52,3fd456332214676c,029d31540328ce6b.html