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国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushrooms.v17.i3.30
pages 231-239

Effects of Ganoderma lucidum (Higher Basidiomycetes) Extracts on the miRNA Profile and Telomerase Activity of the MCF-7 Breast Cancer Cell Line

Oyku Gonul
Department of Medical Biochemistry, Ege University, Bornova, Izmir, Turkey
Hikmet Hakan Aydin
Department of Medical Biochemistry, Ege University School of Medicine, Bornova, Izmir 35100 Turkey
Erbil Kalmis
Ministry of Science, Industry and Technology, Manisa, Turkey
Husniye Kayalar
Department of Pharmacognosy, Ege University, Bornova, Izmir, Turkey
Ali Burak Ozkaya
Department of Medical Biochemistry, Ege University, Bornova, Izmir, Turkey
Sevcan Atay
Department of Medical Biochemistry, Ege University School of Medicine, Bornova, izmir, Turkey
Handan Ak
Department of Medical Biochemistry, Ege University School of Medicine, Bornova, izmir, Turkey

ABSTRACT

Ganoderma lucidum is a medicinal higher Basidiomycetes mushroom that exerts anticancer effects through several different mechanisms. This study investigated the effects of G. lucidum on the telomerase activity and microRNA (miRNA) profiles of MCF-7 cells. According to the cytotoxicity results, the G. lucidum ether extract exhibits the highest cytotoxic potency; therefore it was chosen for the subsequent telomerase activity assay and miRNA profiling. The telomerase activity observed in the cells treated with a half-maximal inhibitory concentration of G. lucidum ether extract (100 µg/mL in dimethyl sulfoxide) was 32.2% lower than that of the control cells treated with 1% dimethyl sulfoxide. Among 1066 miRNAs, the most downregulated miRNA was hsa-miR-27a* (4.469-fold), and the most upregulated miRNA was hsa-miR-1285 (10.462-fold). A database search revealed the predicted miRNAs that target the catalytic subunit of the telomerase enzyme telomerase reverse transcriptase, and only miR-3687 (upregulated 2.153-fold) and miR-1207-5p (upregulated 2.895-fold) were changed by at least 2-fold. The miRNA profile changes demonstrated in this study provide a data set regarding their effects on the pathways that regulate telomerase activity in MCF-7 breast cancer cells treated with G. lucidum. These data should aid the development of novel cancer treatment strategies.