图书馆订阅: Guest
Begell Digital Portal Begell 数字图书馆 电子图书 期刊 参考文献及会议录 研究收集
国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushr.v10.i4.90
pages 361-368

Purification and Particular Characterization of Laccase from the Ling Zhi or Reishi Medicinal Mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. 447 (Aphyllophoromycetideae)

George G. Songulashvili
Institute of Evolution, Department of Evolutionary and Environmental Biology, Faculty of Science and Science Education, University of Haifa, Mt. Carmel, Haifa, 31905 Israel; and Durmishidze Institute of Biochemistry and Biotechnology,0159 Tbilisi, Georgia
Vladimir I. Elisashvili
Animal Husbandry and Feed Production Institute of Agricultural University of Georgia, 240 David Agmashenebeli alley, 0159 Tbilisi, Georgia
Eviatar D. Nevo
Department of Evolutionary and Environmental Biology, Faculty of Natural Sciences, Institute of Evolution, University of Haifa, 199 Abba Khousi Ave., Mt. Carmel, Haifa 3498838, Israel
Yitzhak Hadar
Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 76100, Israel

ABSTRACT

The Ganoderma lucidum strain 447 was cultivated in a 10-L fermentor. We used the ethanol-production residue (by wheat) (REP) as the growth substrate and Cu as an inductor. Ganoderma lucidum was grown for 8 days before laccase reached the highest yield (188,600 U L−1). The purification enzyme appeared as two-laccase isozyme bands on SDS-PAGE. The molecular masses were 43 and 56 kDa by SDS-PAGE. The pH optimum for 2,2'-azino-bis-[3-ethyltiazoline-6-sulfonate] (ABTS) oxidation was 3 in citric/acetic buffers. In this study, the optimum temperature for laccase activity was determined to be 30°C. The kinetic of laccase was experimented on using 12 phenolic substrates. The lowest Km values (0.0048 and 0.005 mM) were found for syringaldazine and ABTS, respectively.


Articles with similar content:

Comparative Study of the Hemagglutinating Activity of Lectins Isolated from Different Developmental Stages of Culinary-Medicinal Oyster Mushroom, Pleurotus ostreatus (Jacq.: Fr.) Kumm. (Agaricomycetideae)
International Journal of Medicinal Mushrooms, Vol.12, 2010, issue 1
Elene Davitashvili, Ekaterine Kapanadze, Eva Kachlishvili, Eka Metreveli, Vladimir I. Elisashvili
Identification and Quantification of Ergothioneine in Cultivated Mushrooms by Liquid Chromatography-Mass Spectroscopy
International Journal of Medicinal Mushrooms, Vol.8, 2006, issue 3
Robert B. Beelman, N. Joy Dubost, Daniel J. Royse, Devin Peterson
Production of Basidiomata and Ligninolytic Enzymes by the Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), in Licuri (Syagrus coronata) Wastes in Brazil
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 12
Luciana Porto de Souza Vandenberghe, Aline Simoes da Rocha Bispo, Helio Mitoshi Kamida, Maria Gabriela Bello Koblitz, Aristoteles Goes-Neto, Thais Almeida de Menezes
Productivity and Antioxidant Activity of Wild, Reconstituted, and Hybrid Strains of the Pink Oyster Mushroom, Pleurotus djamor (Agaricomycetes), from Mexico
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 7
Paula Cecilia Guadarrama-Mendoza, Rogelio Valadez-Blanco, Norma Francenia Santos-Sánchez, Beatriz Hernández-Carlos, Magdalena Paz Oropeza-Guerrero, Raúl Salas-Coronado
Chemical Characterization and In Vitro Antioxidant Activity Evaluation of Polysaccharides from the Fruiting Bodies of the Red Heart Mushroom Phellinus pini (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 3
Peilong Sun, Ruwei Wang, Jiangning Hu, Chen Xing, Kai Yang, Yuezhong Jin