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国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushr.v16.i1.40
pages 37-47

Dichlormethane Extract of the Jelly Ear Mushroom Auricularia auricula-judae (Higher Basidiomycetes) Inhibits Tumor Cell Growth In Vitro

Md.Ahsanur Reza
Department of Physiology and Pharmacology, Faculty of Animal Science and Veterinary Medicine, Patuakhali Science and Technology University (Barisal Campus), Babugonj, Barisal, Bangladesh
Md. Akil Hossain
College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea
Seung-Jin Lee
College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea
Sileshi Belew Yohannes
College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea
Dereje Damte
College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea; Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel
Man-hee Rhee
College of Veterinary Medicine, Kyungpook National University, Daegu, Republic of Korea
Woo-Sik Jo
Department of Agricultural Environment, Gyeongbuk Agricultural Technology Administration, Daegu 702-701, Republic of Korea
Joo-Won Suh
Center for Nutraceutical and Pharmaceutical Materials, Division of Bioscience and Bioinformatics, Myongji University, Namdong, Cheoin-Gu, Yongin, Gyeonggi, Republic of Korea
Seung-Chun Park
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, Kyungpook National University, Daegu 702701, Republic of Korea

ABSTRACT

In this study, a dichloromethane fraction (DCMF) from 70% Auricularia auricula-judae ethanol extract showed the highest level of antitumor activity compared to other solvent fractions (ethyl acetate, butanol, and water). The DCMF was found to have more potent antitumor activity against broncheoalveolar cancer (half maximal inhibitory concentration = 57.2 µg/mL) and gastric cancer cells (half maximal inhibitory concentration = 73.2 µg/mL) compared to the other solvent fractions, although all fractions inhibited the proliferation of the tumor cells in a dose-dependent manner. We further analyzed the DCMF composition by gas chromatography−coupled mass spectroscopy. Based on the results of this analysis, an antitumor active component (diazane) was identified in the DCMF. However, we found that diazane alone had a lower level of antitumor activity than the DCMF. These findings indicate that other unknown components of the DCMF also are responsible for the cytotoxic effects of DCMF against tumor cells. Semiquantitative reverse transcription polymerase chain reaction analysis demonstrated that DCMF induced cytotoxicity or tumor cell apoptosis as a result of the downregulation of Bcl-2 expression and p53 overexpression. Taken together, our study results demonstrated that the DCMF may be used as a functional additive for enhancing antioxidant activities and suppressing tumor growth in the body.


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