每年出版 12 期
ISSN 打印: 1521-9437
ISSN 在线: 1940-4344
Indexed in
Genetic Diversity and Relationships of 24 Strains of Genus Auricularia (Agaricomycetes) Assessed Using SRAP Markers
摘要
Auricularia species are well known for their traditional edible and medicinal importance. In this study, sequence-related amplified polymorphism (SRAP) markers were used to evaluate the genetic diversity and relationships of 24 Auricularia strains obtained from different ecological regions of China. A total of 402 polymorphic loci were detected using 11 SRAP primer combinations with 100% polymorphic loci. The similarity coefficient ranged from 0.662 to 0.903, with an average of 0.73. Cluster analysis using the pair-group method using arithmetic average, based on the data from SRAP loci amplified by 11 primer pairs, showed that 24 Auricularia strains were distinguished into 4 groups with 73% similarity. The results of principal coordinate analysis were in accordance with pair-group method using arithmetic average clustering, and the first, most informative coordinate accounted for 74.38% of all the variations, which indicated a high level of genetic diversity among Auricularia strains. In general, SRAP markers provide a powerful tool to discriminate Auricularia strains and to evaluate their genetic relationships.