图书馆订阅: Guest
Begell Digital Portal Begell 数字图书馆 电子图书 期刊 参考文献及会议录 研究收集
国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushrooms.2020035042
pages 627-639

Comparative Study on Bioactivities from Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), Gives an Insight into the Fermentation Broth Showing Greater Antioxidative Activities

Chunliang Xie
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Pingping Tang
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China; Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University, Changsha, P.R. China
Shaowei Yan
Hunan Yujia Cosmetics Manufacturing Co. Ltd., No. 668 Qingshan Road, High-Tech Development Zone, Changsha, P.R. China
Qi Yang
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Zhoumei Zhang
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Wenbing Gong
Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, People's Republic of China; Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, People's Republic of China
Zuohua Zhu
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Yingjun Zhou
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Li Yan
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Zhenxiu Hu
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China
Xianchun Wang
Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University, Changsha, P.R. China
Yuande Peng
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R. China

ABSTRACT

Ganoderma lucidum is one of the most famous mushrooms in traditional Chinese medicine. At present, the fully utilized parts of G. lucidum are mainly fruiting body and spore powder. The wild and artificially cultivated G. lucidum fruiting body is costly and rare. Therefore, how to improve the utilization of G. lucidum by means of fermentation is worth investigating. The present study was to perform submerged fermentation of G. lucidum and compare the bioactivities of G. lucidum submerged fermentation broth and fruiting body extract. After the extraction and submerged fermentation methods were optimized, the optimum conditions for extraction were determined as ethanol extraction at 80°C with a solid-to-liquid ratio of 1:30, and those for submerged fermentation were cultivation on malt extract medium for 6 days at 30°C. Under the optimum conditions, the antioxidative activity and tyrosinase inhibition rate of the fermentation broth were 1.2-4.1 fold higher than those of the ethanol extract. Cytotoxicity analysis showed that the ethanol and water extracts and the fermentation broth effectively inhibited pancreatic cancer cells and prostate cancer cells, with much smaller effect on nontumor human embryonic kidney (HEK293T). These results demonstrate that the submerged fermentation could improve the utilization value of G. lucidum and the fermentation broth can be used as an antioxidant additive applied in food, drugs, and cosmetics.

REFERENCES

  1. Sanodiya BS, Thakur GS, Baghel RK, Prasad GB, Bisen PS. Ganoderma lucidum: A potent pharmacological macrofungus. Curr Pharm Biotechnol. 2009;10(8):717-42.

  2. Zolj S, Smith MP, Goines JC, Ali TS, Huff MO, Robinson DL, Lau JM. Antiproliferative effects of a triterpene-enriched extract from Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (Agaricomycetes), on human lung cancer cells. Int J Med Mushrooms. 2018;20(12):1173-83.

  3. Lou HW, Guo XY, Zhang XC, Guo LQ, Lin JF. Optimization of cultivation conditions of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (Agaricomycetes) for the highest antioxidant activity and antioxidant content. Int J Med Mushrooms. 2019;21(4):353-66.

  4. Chen SD, Yong TQ, Zhang YF, Hu HP, Xie YZ. Inhibitory effect of five Ganoderma species (Agaricomycetes) against key digestive enzymes related to Type 2 diabetes mellitus. Int J Med Mushrooms. 2019;21(7):703-11.

  5. Zhang J, Meng G, Zhai G, Yang Y, Zhao H, Jia L. Extraction, characterization and antioxidant activity of polysaccharides of spent mushroom compost of Ganoderma lucidum. Int J Biol Macromol. 2016;82(2):432-439.

  6. Zhonghui Z, Xiaowei Z, Fang F. Ganoderma lucidum polysaccharides supplementation attenuates exercise-induced oxidative stress in skeletal muscle of mice. Saudi J Biol Sci. 2014;21(2):119-23.

  7. Qin Y, Xiong L, Li M, Liu J, Wu H, Qiu H, Mu H, Xu X, Sun Q. Preparation of bioactive polysaccharide nanoparticles with enhanced radical scavenging activity and antimicrobial activity. J Agric Food Chem. 2018;66(17):4373-83.

  8. Lo YC, Chien SC, Mishchuk DO, Slupsky CM, Mau JL. Quantification of water-soluble metabolites in medicinal mushrooms using proton NMR spectroscopy. Int J Med Mushrooms. 2016;18(5):413-24.

  9. Ofodile LN, Uma NU, Kokubun T, Grayer RJ, Ogundipe OT, Simmonds MSJ. Antimicrobial activity of some Ganoderma species from Nigeria. Phytother Res. 2010;19(4):310-3.

  10. Sun YX, Liu JC, Kennedy JF. Purification, composition analysis and antioxidant activity of different polysaccharide conjugates (APPs) from the fruiting bodies of Auricularia polytricha. Carbohydr Polym. 2010;82(2):299-304.

  11. Ozcariz-Fermoselle MV, Fraile-Fabero R, Girbes-Juan T, Arce-Cervantes O, Oria de Rueda-Salgueiro JA, Azul AM. Use of lignocellulosic wastes of pecan (Carya illinoinensis) in the cultivation of Ganoderma lucidum. Rev Iberoam Micol. 2018;35(2):103-9.

  12. Antecka A, Bizukojc M, Ledakowicz S. Modern morphological engineering techniques for improving productivity of filamentous fungi in submerged cultures. World J Microbiol Biotechnol. 2016;32(12):193-202.

  13. Zhang YP, Sun J, Ma Y. Biomanufacturing: History and perspective. J Ind Microbiol Biotechnol. 2017;44(4-5):773-84.

  14. Bizukojc M, Ledakowicz S. Bioprocess engineering aspects of the cultivation of a Lovastatin producer Aspergillus terreus. Adv Biochem Eng Biotechnol. 2015;149:133-70.

  15. Cizmarikova M. The efficacy and toxicity of using the Lingzhi or Reishi Medicinal mushroom, Ganoderma lucidum (Agaricomycetes), and its products in chemotherapy: A review. Int J Med Mushrooms. 2017;19(10):861-77.

  16. Boh B. Ganoderma lucidum: A potential for biotechnological production of anticancer and immunomodulatory drugs. Recent Pat Anticancer Drug Discov. 2013;8(3):255-87.

  17. Wang CH, Hsieh SC, Wang HJ, Chen ML, Lin BF, Chiang BH, Lu TJ. Concentration variation and molecular characteristics of soluble (1,3;1,6)-beta-D-glucans in submerged cultivation products of Ganoderma lucidum mycelium. J Agric Food Chem. 2014;62(3):634-41.

  18. Zhou S, Tang Q, Tang C, Liu Y, Ma F, Zhang X, Zhang JS. Triterpenes and soluble polysaccharide changes in lingzhi or reishi medicinal mushroom, Ganoderma lucidum (Agaricomycetes), during fruiting growth. Int J Med Mushrooms. 2018;20(9):859-71.

  19. Ye L, Liu S, Xie F, Zhao L, Wu X. Enhanced production of polysaccharides and triterpenoids in Ganoderma lucidum fruit bodies on induction with signal transduction during the fruiting stage. PLoS One. 2018;13(4):e0196287.

  20. Sudheer S, Alzorqi I, Ali A, Cheng PG, Siddiqui Y, Manickam S. Determination of the biological efficiency and antioxidant potential of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (Agaricomycetes), cultivated using different agro-wastes in Malaysia. Int J Med Mushrooms. 2018;20(1):89-100.

  21. Bidegain MA, Postemsky PD, Pieroni O, Cubitto MA. Analysis of the influence of substrate formulations on the bioactive chemical profile of Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (Agaricomycetes) by conventional and chemometrics methods. Int J Med Mushrooms. 2019;21(6):537-48.

  22. Zou Y, Hou X. Extraction optimization, composition analysis, and antioxidation evaluation of polysaccharides from white jelly mushroom, Tremella fuciformis (Tremellomycetes). Int J Med Mushrooms. 2017;19(12):1113-21.

  23. Xin H, Fang L, Xie J, Qi W, Niu Y, Yang F, Cai D, Zhang Y, Wen Z. Identification and quantification of triterpenoids in lingzhi or reishi medicinal mushroom, Ganoderma lucidum (Agaricomycetes), with HPLC-MS/MS methods. Int J Med Mushrooms. 2018;20(10):919-34.

  24. Lu SF, Shuchi T, Lin HC, Fu WG. Study on the analytical method for water-soluble crude polysaccharides in culinary-medicinal mushrooms. Int J Med Mushrooms. 2010;12(4):331-45.

  25. Berovic M, Podgornik BB. Engineering aspects of Lingzhi or Reishi medicinal mushroom Ganoderma lucidum (Agaricomycetes) biomass submerged cultivation in bioreactors: A review. Int J Med Mushrooms. 2019;21(3):253-65.

  26. Milovanovic I, Stajic M, Stanojkovic T, Knezevic A, Vukojevic J. Effects of selenium presence in mycelia of Ganoderma species (higher Basidiomycetes) on their medicinal properties. Int J Med Mushrooms. 2015;17(1):11-20.

  27. Asatiani MD, Elisashvili VI, Wasser SP, Reznick AZ, Nevo E. Free-radical scavenging activity of submerged mycelium extracts from higher Basidiomycetes mushrooms. Biosci Biotechnol Biochem. 2007;71(12):3090-2.

  28. Cilerdzic J, Vukojevic J, Stajic M, Stanojkovic T, Glamoclija J. Biological activity of Ganoderma lucidum basidiocarps cultivated on alternative and commercial substrate. J Ethnopharmacol. 2014;155(1):312-9.

  29. Hsu KD, Chen HJ, Wang CS, Lum CC, Wu SP, Lin SP, Cheng KC. Extract of Ganoderma formosanum mycelium as a highly potent tyrosinase inhibitor. Sci Rep. 2016;6:32854.

  30. Liang Z, Ding Z, Peng X, Wang Y, Gu Z, Zhu Q, Shi G, Zhang K. Methyl lucidenate F isolated from the ethanol-soluble-acidic components of Ganoderma lucidum is a novel tyrosinase inhibitor. Biotechnol Bioprocess Eng. 2011;16(3):457.

  31. Hirotani M, Furuya T, Shiro M. A ganoderic acid derivative, a highly oxygenated lanostane-type triterpenoid from Ganoderma lucidum. Phytochemistry. 1985;24(9):2055-61.

  32. Liu GQ, Wang XL, Zhang YG, Wu YH, Han WJ, Zhang HY. Promotion of ganoderic acid production in Ganoderma sinense by the addition of an ether extract from Eupolyphaga sinensis, a medicinal insect. African J Biotechnol. 2010;9(37):6129-34.


Articles with similar content:

Extraction, Antimicrobial, and Antioxidant Activities of Crude Polysaccharides from the Wood Ear Medicinal Mushroom Auricularia auricula-judae (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 6
Peilong Sun, Yang Lin, Han-hua Liang, Yin-long Luo, Ming Cai
Characterization of Anti−Salmonella typhi Compounds from Medicinal Mushroom Extracts from Zimbabwe
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 7
Catherine Chidewe, Mudadi A. Benhura, Tsungai Reid, Chenjerayi Kashangura, Takafira Mduluza, Babill Stray-Pedersen
Effects of Various Treatments on Extraction of the Main Bioactive Components and Determination of Biological Activity of Extracts from the Caterpillar Medicinal Mushroom Cordyceps militaris (Ascomycetes)
International Journal of Medicinal Mushrooms, Vol.20, 2018, issue 9
Chaomin Yin, Hong Gao, Xiuzhi Fan, De-Fang Shi, Zhe Fan
Screening for Antimicrobial Activity of Wood Rotting Higher Basidiomycetes Mushrooms from Uruguay against Phytopathogens
International Journal of Medicinal Mushrooms, Vol.18, 2016, issue 3
Alvaro Vázquez, María Pía Cerdeiras, Gianna Cecchetto, Stephanie Barneche, Gabriela Jorcin, Silvana Alborés
Antibacterial Properties of Selected Lignicolous Mushrooms and Fungi from Northern Serbia
International Journal of Medicinal Mushrooms, Vol.11, 2009, issue 3
Petar Knezevic, Neda Mimica-Dukic, Milan Matavuly, Maja Karaman, Zorica Svircev