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国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.431 SNIP: 0.716 CiteScore™: 2.6

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushr.v11.i4.20
pages 351-364

Anticancer Effect of Fraction Isolated from Medicinal Birch Polypore Mushroom, Piptoporus betulinus (Bull.: Fr.) P. Karst. (Aphyllophoromycetideae): In Vitro Studies

Marta Kinga Lemieszek
Department of Medical Biology, Institute of Rural Health, Lublin, Poland
Ewa Langner
Department of Medical Biology, Institute of Agricultural Medicine; and Department of Virology and Immunology, Institute of Microbiology and Biotechnology, Maria Curie-Skіodowska University, Lublin, Poland
Jozef Kaczor
Department of Virology and Immunology, Institute of Microbiology and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin
Martyna Kandefer-Szerszen
Department of Virology and Immunology, Institute of Microbiology and Biotechnology, Maria Curie-Sklodowska University, Akademicka 19, 20-033 Lublin
Bozena Sanecka
Department of Biochemistry and Biotechnology, Faculty of Chemistry, Rzeszów University of Technology, Al. Powstanców Warszawy 6, 35-959 Rzeszów
Witold Mazurkiewicz
Department of Organic Chemistry, Faculty of Chemistry, Rzeszów University of Technology, Al. Powstanców Warszawy 6, 35-959 Rzeszów
Wojciech Rzeski
Department of Medical Biology, Institute of Rural Health, Lublin, Poland; Department of Virology and Immunology, Maria Curie-Sklodowska University, Lublin, Poland

ABSTRACT

Piptoporus betulinus (Bull.: Fr.) P. Karst. (Fomitopsidaceae) has been commonly used in folk medicine as an antiparasitic and antimicrobial agent in the treatment of wounds and for the treatment of rectal cancer and stomach diseases. Tea obtained from this mushroom has antibacterial, antifatiguing, immunoenhancing, and soothing properties. The purpose of the present study was the evaluation of in vitro anticancer activity of fraction isolated from P. betulinus. The effect on cell proliferation, motility, and viability was assessed in a range of cancer and normal cells. P. betulinus fraction prepared from dried fruiting bodies was subjected to anticancer evaluation in human lung carcinoma (A549), colon adenocarcinoma (HT-29), and rat glioma (C6) cell cultures. Human skin fibroblasts (HSF), bovine aorta endothelial cells (BAEC), models of rat oligodenrocytes (OLN-93), hepatocytes (Fao), rat astroglia, and mouse neurons (P19) were applied to test toxicity in normal cells. The following methods were applied: tumor cell proliferation (MTT assay and BrdU assay), cytotoxicity (LDH assay), tumor cell motility (wound assay), tumor cell morphology (May-Grünwald-Giemsa staining), and death detection (ELISA). P. betulinus fraction elicited anticancer effects that were attributed to decreased tumor cell proliferation, motility, and the induction of morphological changes. Of note is the fact that it produced no or low toxicity in tested normal cells.


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