图书馆订阅: Guest
Begell Digital Portal Begell 数字图书馆 电子图书 期刊 参考文献及会议录 研究收集
国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.433 SNIP: 0.661 CiteScore™: 1.38

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushrooms.2017024487
pages 1029-1040

Identification of Reference Genes and Analysis of Heat Shock Protein Gene Expression in Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum, after Exposure to Heat Stress

Yong-Nan Liu
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing, People's Republic of China
Xiao-Xiao Lu
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing, People's Republic of China
Ang Ren
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing, People's Republic of China
Liang Shi
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing, People's Republic of China
Ai-Liang Jiang
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing, People's Republic of China
Han-Shou Yu
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing, People's Republic of China
Ming-Wen Zhao
Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Microbiology Department, College of Life Sciences, Nanjing Agricultural University, Jiangsu, Nanjing, People's Republic of China

ABSTRACT

Ganoderma lucidum has been considered an emerging model species for studying how environmental factors regulate the growth, development, and secondary metabolism of Basidiomycetes. Heat stress, which is one of the most important environmental abiotic stresses, seriously affects the growth, development, and yield of microorganisms. Understanding the response to heat stress has gradually become a hotspot in microorganism research. But suitable reference genes for expression analysis under heat stress have not been reported in G. lucidum. In this study, we systematically identified 11 candidate reference genes that were measured using reverse transcriptase quantitative polymerase chain reaction, and the gene expression stability was analyzed under heat stress conditions using geNorm and NormFinder. The results show that 5 reference genes−CYP and TIF, followed by UCE2, ACTIN, and UBQ1−are the most stable genes under our experimental conditions. Moreover, the relative expression levels of 3 heat stress response genes (hsp17.4, hsp70, and hsp90) were analyzed under heat stress conditions with different normalization strategies. The results show that use of a gene with unstable expression (SAND) as the reference gene leads to biased data and misinterpretations of the target gene expression level under heat stress.


Articles with similar content:

Identification and Evaluation of Reference Genes for qRT-PCR Normalization in Sparassis latifolia (Agaricomycetes)
International Journal of Medicinal Mushrooms, Vol.21, 2019, issue 3
Yanquan Lin, Lu Ma, Xiaoling Jiang, Chi Yang, Zhenghe Ying, Donglai Xiao
A Common Regulator of Cold and Radiation Response in Escherichia coli
Journal of Environmental Pathology, Toxicology and Oncology, Vol.20, 2001, issue 1
Vibhav R. Sanzgiri, Suhas Mangoli, Suresh K. Mahajan
c-Myc-Induced Genomic Instability
Journal of Environmental Pathology, Toxicology and Oncology, Vol.22, 2003, issue 3
Sabine Mai, J. Frederic Mushinski
P-SOFT ROUGH FUZZY GROUPS AND P-SOFT FUZZY ROUGH GROUPS AND CORRESPONDING DECISION-MAKING
International Journal for Uncertainty Quantification, Vol.8, 2018, issue 1
Jianming Zhan, Wenjun Pan
Bioinformatic Identification of Potential MicroRNAs and Their Targets in the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Higher Basidiomycetes)
International Journal of Medicinal Mushrooms, Vol.17, 2015, issue 8
Ang Ren, Ming-Wen Zhao, Dashuai Mu, Xuchen Zhang, Liang Shi, Chenyang Li