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国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/InterJMedicMush.v5.i2.50
6 pages

Purification of Caffeic Acid as an Antioxidant from Submerged Culture Mycelia of Phellinus linteus (Berk. et Curt.) Teng (Aphyllophoromycetideae)

Tomoyuki Nakamura
Applied Fungi Institute, IBI Corporation, 7841 Anayama-cyo, Nirasaki-shi, Yamanashi 407-0263; and Faculty of Engineering, Yamanashi University, 4-3-11 Takeda, Kofu-shi, Yamanashi 400-8511, Japan
Yukihito Akiyama
Applied Fungi Institute, IBI Corporation, 7841 Anayama-cyo, Nirasaki-shi, Yamanashi 407-0263, Japan
Seiichi Matsugo
Faculty of Engineering, Yamanashi University, 4-3-11 Takeda, Kofu-shi, Yamanashi 400-8511, Japan
Yasuyuki Uzuka
Faculty of Engineering, Yamanashi University, 4-3-11 Takeda, Kofu-shi, Yamanashi 400-8511, Japan
Keiji Shibata
Faculty of Agriculture, Shizuoka University, 836 Ohya, Shizuoka 422-8529, Japan
Hirokazu Kawagishi
Research Institute of Green Science and Technology, Shizuoka University, Shizuoka, Japan; Graduate School of Agriculture, Shizuoka University, Shizuoka, Japan; and Graduate School of Science and Technology, Shizuoka University, Japan

ABSTRACT

Phellinus linteus mycelia have many pharmacological effects although their pharmacological efficacy principles have not been known before. In the course of screening for biological activity of the extracts of Ph. Linteus mycelia, we found strong antioxidative activity in the extract. Therefore, we tried to isolate the active principle(s) from the extract. The isolation of the active compound was guided by superoxide anion radical scavenging activity. As a result, caffeic acid was isolated as an active compound. The IC50 of the compound was 3.05 μg/mL (16.9 μM).


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