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国际药用蘑菇期刊
影响因子: 1.423 5年影响因子: 1.525 SJR: 0.431 SNIP: 0.661 CiteScore™: 1.38

ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushr.v7.i12.230
pages 243-248

Lectin from Tricholoma mongolicum S. Imai (Agaricomycetideae) Mycelia Stimulates Gene Expression of Immunomodulating Cytokines in Mouse Peritoneal Macrophages and Splenocytes

Fang Liu
Department of Microbiology, College of Life Science, Nankai University, Tianjin, China
Tzi Bun Ng
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China
Hexiang Wang
Department of Microbiology, China Agricultural University, Beijing, China
Ming Chiu Fung
Department of Biology, The Chinese University of Hong Kong, Shatin, Hong Kong, China
Vincent Eng Choo Ooi
Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories Hong Kong, China

ABSTRACT

The effects of lectin from the edible mushroom Tricholoma mongolicum on cytokine gene expression were studied. RNA was extracted from splenocytes and peritoneal exudate cells 24 hours after the last of a series of 10 daily intraperitoneal injections (10 μg/mouse/injection), and reverse transcription/polymerase chain reaction was employed in the analysis of cytokine gene expression. In peritoneal exudate cells, the level of gene expression of inducible nitric oxide synthetase, interleukin (IL)-1β, and transforming growth factor-β was upregulated by the lectin. In spleen cells, the level of gene expression of inducible IL-1β was upregulated.