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国际药用蘑菇期刊
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ISSN 打印: 1521-9437
ISSN 在线: 1940-4344

国际药用蘑菇期刊

DOI: 10.1615/IntJMedMushr.v6.i1.50
8 pages

Immune-Competent Polysaccharides from the Submerged Cultured Mycelium of Culinary—Medicinal Mushroom Lentinus strigellus Berk. et Curt. (Agaricomycetideae)

Yuexin Lin
The College of Bioengineering, Fujian Normal University, Fuzhou, R.P. China
Ping Lai
The College of Bioengineering, Fujian Normal University, Fuzhou, R.P. China
Yanyan Huang
The College of Bioengineering, Fujian Normal University, Fuzhou, R.P. China
Hualing Xie
The College of Bioengineering, Fujian Normal University, Fuzhou, R.P. China

ABSTRACT

Two main fractions of polysaccharide SL-I and SL-II-b were purified from submerged cultured mycelium of culinary—medicinal mushroom Lentinus strigellus using hot water extraction, ethanol precipitation, DEAE-cellulose chromatography, and Sephadex G-100 filtration. Polysaccharide analysis showed that SL-I is a protein-free polysaccharide with 1.15 × 104 Da molecular weight and +63.33° optical rotation, while SL-II-b is a protein-conjugated polysaccharide with 3.88 × 103 Da molecular weight and +73.67° optical rotation. The sugar constituents of polysaccharide analysis by GC showed that both fractions are heteropolysaccharides. SL-I consists of six monosaccharides, and SL-II-b consists of three monosaccharides. IR and 1H-NMR, 13C-NMR spectra further indicated that LS-I and LS-II-b are heteropolysaccharides with a(1®2),(1®4) glucosidic bonds (LS-I), and a(1®2),(1®3),(1®4) glucosidic bonds (LS-II-b). The immune-competent examination showed that both polysaccharides could increase the spleen index, carbon clearance index, phagocytic index, and white blood cell levels of CY-treated mice.


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