卷 5,
册 2, 2014,
pp. 111-119
DOI: 10.1615/IntJPhysPathophys.v5.i2.20
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Victoria V. Nikulina
NSC "Institute of Biology", Kyiv National Taras Shevchenko University, Kiev, Ukraine
Liudmyla V. Garmanchuk
ESC "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv 03127, Ukraine
Alexandr N. Makarenko
The Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia
Nataliya N. Khranovskaya
National Cancer Institute, Kiev, Ukraine
Tetiana V. Nikolaienko
NSC "Institute of Biology", Kyiv National Taras Shevchenko University, Kiev, Ukraine
Kristina D. Nepiyvoda
NSC "Institute of Biology", Kyiv National Taras Shevchenko University, Kiev, Ukraine
Liudmyla I. Ostapchenko
NSC "Institute of Biology", Kyiv National Taras Shevchenko University, Kiev, Ukraine
Sergey G. Morozov
Institute of General Pathology and Pathophysiology, Russian Academy of Sciences, Moscow, Russia
Nikolay S. Kositsyn
The Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow, Russia; Institute of General Pathology and Pathophysiology, Russian Academy of Sciences, Moscow, Russia
摘要
The effect of mitocorrectin (complex of native oligopeptides isolated from the brain of newborn pigs) on the endothelial cells in culture was studied. The preparation has been revealed to induce angiogenesis in vitro in a concentration-dependent manner. Based on the results of MTT assay and a standard cell counting in Goryaev chamber, mitogenic effect of mitocorrectin was shown at concentration of 0.1 − 1 mg / ml, which involves an increasey in the number of cells by 25% ± 5%, as well as increased subpopulations of proliferative cell pool (G2 / M + S) 1.8-fold after mitocorrectin dilutions from 0.01 to 0.05 mg / ml, relative to the control value. In 3D-cultured cells and in the stationary growth, effects were fixed which indicate mitocorrectin-induced differentiation of the endothelial cells: reduced NO production, increased metabolism of glucose, and the formation of capillary-like structures.