图书馆订阅: Guest
Begell Digital Portal Begell 数字图书馆 电子图书 期刊 参考文献及会议录 研究收集
真核基因表达评论综述™
影响因子: 1.841 5年影响因子: 1.927 SJR: 0.627 SNIP: 0.516 CiteScore™: 1.96

ISSN 打印: 1045-4403
ISSN 在线: 2162-6502

真核基因表达评论综述™

DOI: 10.1615/CritRevEukaryotGeneExpr.2019028155
pages 123-126

Antioxidant Enzymes Expression of the Impaired Contralateral Testes in Unilaterally Cryptorchid Rats

Bao-Ping Zhu
Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China
Liang Cai
Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan, China

ABSTRACT

Objective: In this study, explored the pathologic mechanism of the contralateral testes impairment in unilaterally cryptorchid rats by investigating the gene expression level. Methods: Thirty male Sprague-Dawley rats were randomly and evenly divided into two groups: cryptorchid group and control group. Cryptorchidism was induced by surgical relocation. RT-PCR was then applied to examine the mRNA expression level of antioxidant enzymes in descended testes, including glutathione peroxidase (GSH-PX), copper/zinc-superoxide dismutase (Cu/Zn-SOD), and catalase (CAT). The concentration of malondialdehyde (MDA) was determined by spectrophotometry. In addition, germ-cell apoptosis was detected by a terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay. Results: Two weeks after the operation, the mRNA expression levels of GSH-PX and Cu/Zn-SOD in the cryptorchid group were significantly downregulated; the expression of MDA, as well as the number of apoptotic germ cells, significantly increased compared to the control group (p < 0.01). The mRNA expression of CAT did not show significant changes (p > 0.05). Conclusion: GSH-PX and SOD were downregulated in the testis contralateral to the undescended testis, leading to the accumulation of reactive oxygen species and germ-cell apoptosis. Our results may provide molecular explanations for the impairment of the descended testis in unilateral cryptorchidism.

REFERENCES

  1. Hutson JM, Balic A, Nation T, Southwell B. , Cryptorchidism. Semin Pediatr Surg. 2010;19:215–24.

  2. Virtanen HE, Toppari J. , Cryptorchidism and fertility. Endocrinol Metab Clin North Am. 2015;44:751–60.

  3. Group UKTCS. Aetiology of testicular cancer: association with congenital abnormalities, age at puberty, infertility, and exercise. U K Test Cancer Study Group. BMJ. 1994;308:1393–99.

  4. Cortes D. , Cryptorchidism–aspects of pathogenesis, histology and treatment. Scand J Urol Nephrol Suppl. 1998;196:1–54.

  5. Bergh A, Soder O. , Studies of cryptorchidism in experimental animal models. Acta Paediatr. 2007;96:617–21.

  6. Rio DC, Ares M, Jr., Hannon GJ, Nilsen TW. , Purification of RNA using TRIzol (TRI reagent). Cold Spring Harb Protoc. 2010;2010:pdb prot5439.

  7. Khoubnasabjafari M, Ansarin K, Jouyban A. , Reliability of malondialdehyde as a biomarker of oxidative stress in psychological disorders. Bioimpacts. 2015;5:123–27.

  8. Kyrylkova K, Kyryachenko S, Leid M, Kioussi C. , Detection of apoptosis by TUNEL assay. Method Mol Biol. 2012;887:41–47.

  9. Foresta C, Ferlin A, Moro E, Garolla A, Rossato M, Scandellari C. , Microdeletions of the Y chromosome in cryptorchidism and in idiopathic male infertility. Ann Ital Med Int. 1999;14:151–58.

  10. Lenzi A, Gandini L, Lombardo F, Cappa M, Nardini P, Ferro F, Borrelli P, Dondero F. , Antisperm antibodies in young boys. Andrologia. 1991;23:233–35.

  11. Pinart E, Sancho S, Briz MD, Bonet S, Garcia N, Badia E. , Ultrastructural study of the boar seminiferous epithelium: changes in cryptorchidism. J Morphol. 2000;244:190–202.

  12. Zhu XS, Zheng XM, Li SW, Zheng H, Hu LQ. , Investigation of the mechanism of genitofemoral nerve in the contralateral testes damage of unilateral cryptorchid rats. Zhonghua Nan Ke Xue. 2002;8:264–65.

  13. Peltola V, Huhtaniemi I, Ahotupa M. , Antioxidant enzyme activity in the maturing rat testis. J Androl. 1992;13:450–55.

  14. Zini A, Schlegel PN. , Expression of glutathione peroxidases in the adult male rat reproductive tract. Fertil Steril. 1997;68:689–95.

  15. Zini A, Schlegel PN. , Effect of hormonal manipulation on mRNA expression of antioxidant enzymes in the rat testis. J Urol. 2003;169:767–71.

  16. Jana K, Dutta A, Chakraborty P, Manna I, Firdaus SB, Bandyopadhyay D, Chattopadhyay R, Chakravarty B. , Alpha-lipoic acid and N-acetylcysteine protects intensive swimming exercise-mediated germ-cell depletion, pro-oxidant generation, and alteration of steroidogenesis in rat testis. Mol Reprod Dev. 2014;81:833–50.

  17. Treulen F, Uribe P, Boguen R, Villegas JV. , Mitochondrial permeability transition increases reactive oxygen species production and induces DNA fragmentation in human spermatozoa. Hum Reprod. 2015;30:767–76.

  18. Kaur S, Bansal MP. , Protective role of dietary-supplemented selenium and vitamin E in heat-induced apoptosis and oxidative stress in mice testes. Andrologia. 2015;47:1109–19.

  19. Evangelista-Vargas S, Santiani A. , Detection of intracellular reactive oxygen species (superoxide anion and hydrogen peroxide) and lipid peroxidation during cryopreservation of alpaca spermatozoa. Reprod Domest Anim. 2017;52:819–24.


Articles with similar content:

Protective Effect of Troxerutin on Nickel-Induced Testicular Toxicity in Wistar Rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.35, 2016, issue 2
Ramalingam Ramakrishnan, Abdulkadhar Mohamed Jalaludeen, Leelavinothan Pari, Perumal Elangovan
Ameliorative Effect of Green Tea Catechin Against Cadmium Chloride−Induced Testicular Toxicity in Mice
Journal of Environmental Pathology, Toxicology and Oncology, Vol.34, 2015, issue 4
Priyanka Sharma, Pradeep Kumar Goyal
Zoledronic Acid Aggravates Kidney Damage During Ischemia Reperfusion Injury in Rat
Journal of Environmental Pathology, Toxicology and Oncology, Vol.34, 2015, issue 1
Orhan Unal Zorba, Levent Tumkaya, Ahmet Fikret Yucel, Ibrahim Sehitoglu, Medine Cumhur Cure, Erkan Cure, Suleyman Yuce, Recep Bedir, Yildiray Kalkan
Hepatoprotective and Immunosuppressive Effect of Synedrella nodiflora L. on Carbon Tetrachloride (CCl4)-Intoxicated Rats
Journal of Environmental Pathology, Toxicology and Oncology, Vol.35, 2016, issue 1
Muhammad Dawood Shah, A.T.M. Emdadul Haque, Mohammad Iqbal, Jaafar Sadeq Makki, Charles Gnanaraj
Role of Zinc in Modulating Histoarchitectural and Biochemical Alterations During Dimethylhydrazine (DMH)-Induced Rat Colon Carcinogenesis
Journal of Environmental Pathology, Toxicology and Oncology, Vol.28, 2009, issue 4
Devinder K. Dhawan, Anshoo Malhotra, Vijayta Dani Chadha, Praveen Nair