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ISSN 打印: 1947-5764

ISSN 在线: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Proteomic Tools to Characterize Non-Thermal Plasma Effects in Eukaryotic Cells

卷 3, 册 1-2, 2013, pp. 81-95
DOI: 10.1615/PlasmaMed.2014009690
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摘要

Plasma medicine is an exciting new scientific field due to recent developments in nonthermal physical plasmas operating at atmospheric pressure. In the present study, the effect of an argon-operated plasma jet (kINPen) using either humidified or dry argon as the working gas was investigated on human keratinocytes with respect to changes in the cellular protein expression pattern. The possibility of characterizing the plasma source by its effects on the cell model was tested. After successfully establishing the gel-free proteomics approach using liquid chromatography−high-resolution mass spectrometry, a data set of 3,818 different human proteins from all cellular compartments and protein classes was analyzed. Overall, 10% of proteins were regulated by the plasma treatment, indicating a strong effect of the plasma on the human cell. While there is only weak evidence for direct protein modification, plasma does trigger the active translation of stress-responding proteins. Among the most regulated proteins, cytoskeletal components (keratins), chaperones (heat shock proteins), and proteins involved in oxygen turnover (oxidoreductases, NQO1) were found. Therefore, the presence of reactive oxygen species as well as an organized cellular response are indicated, emphasizing the need for further research in medical applications. Additionally, our approach enables the differentiation between the two selected plasma parameters, allowing its further use to identify key players both in plasma-treated liquids and cellular response. This study and the methodology described herein can be used as a basis to further address the underlying mechanisms of plasma−cell interactions. The data obtained will facilitate fundamental understanding on cellular responses after plasma stimulation.

对本文的引用
  1. Bekeschus Sander, Schmidt Anke, Bethge Lydia, Masur Kai, von Woedtke Thomas, Hasse Sybille, Wende Kristian, Redox Stimulation of Human THP-1 Monocytes in Response to Cold Physical Plasma, Oxidative Medicine and Cellular Longevity, 2016, 2016. Crossref

  2. Hänsch Mareike A C, Mann Miriam, Weltmann Klaus-Dieter, von Woedtke Thomas, Analysis of antibacterial efficacy of plasma-treated sodium chloride solutions, Journal of Physics D: Applied Physics, 48, 45, 2015. Crossref

  3. Kluge Susanne, Bekeschus Sander, Bender Claudia, Benkhai Hicham, Sckell Axel, Below Harald, Stope Matthias B., Kramer Axel, Yousfi Mohammed, Investigating the Mutagenicity of a Cold Argon-Plasma Jet in an HET-MN Model, PLOS ONE, 11, 9, 2016. Crossref

  4. Schmidt Anke, von Woedtke Thomas, Bekeschus Sander, Periodic Exposure of Keratinocytes to Cold Physical Plasma: AnIn VitroModel for Redox-Related Diseases of the Skin, Oxidative Medicine and Cellular Longevity, 2016, 2016. Crossref

  5. Kramer Axel, Bekeschus Sander, Matthes Rutger, Bender Claudia, Stope Matthias B., Napp Matthias, Lademann Olaf, Lademann Jürgen, Weltmann Klaus-Dieter, Schauer Frieder, Cold Physical Plasmas in the Field of Hygiene-Relevance, Significance, and Future Applications, Plasma Processes and Polymers, 12, 12, 2015. Crossref

  6. Schmidt Anke, Rödder Katrin, Hasse Sybille, Masur Kai, Toups Lena, Lillig Christopher H., von Woedtke Thomas, Wende Kristian, Bekeschus Sander, Redox-regulation of activator protein 1 family members in blood cancer cell lines exposed to cold physical plasma-treated medium, Plasma Processes and Polymers, 13, 12, 2016. Crossref

  7. Schmidt Anke, Bekeschus Sander, Wende Kristian, Vollmar Brigitte, von Woedtke Thomas, A cold plasma jet accelerates wound healing in a murine model of full-thickness skin wounds, Experimental Dermatology, 26, 2, 2017. Crossref

  8. Bekeschus Sander, Schmidt Anke, Jablonowski Helena, Bethge Lydia, Hasse Sybille, Wende Kristian, Masur Kai, von Woedtke Thomas, Weltmann Klaus-Dieter, Environmental Control of an Argon Plasma Effluent and Its Role in THP-1 Monocyte Function, IEEE Transactions on Plasma Science, 45, 12, 2017. Crossref

  9. Classen Janine, Dengler Berrett, Klinger Christoph, Bettenay Sonya, Rickerts Volker, Mueller Ralf, Cutaneous alternariosis in an immunocompromised dog successfully treated with cold plasma and cessation of immunosuppressive medication, Tierärztliche Praxis Ausgabe K: Kleintiere / Heimtiere, 45, 05, 2017. Crossref

  10. Umair Muhammad, Jabbar Saqib, Ayub Zubaria, Muhammad Aadil Rana, Abid Muhammad, Zhang Jianhao, Liqing Zhao, Recent Advances in Plasma Technology: Influence of Atmospheric Cold Plasma on Spore Inactivation, Food Reviews International, 2021. Crossref

  11. Schmidt Anke, Dietrich Stephan, Steuer Anna, Weltmann Klaus-Dieter, von Woedtke Thomas, Masur Kai, Wende Kristian, Non-thermal Plasma Activates Human Keratinocytes by Stimulation of Antioxidant and Phase II Pathways, Journal of Biological Chemistry, 290, 11, 2015. Crossref

  12. Bhartiya Pradeep, Kaushik Neha, Nguyen Linh N., Bekeschus Sander, Masur Kai, Weltmann Klaus-Dieter, Kaushik Nagendra Kumar, Choi Eun Ha, Periodic Exposure of Plasma-Activated Medium Alters Fibroblast Cellular Homoeostasis, International Journal of Molecular Sciences, 23, 6, 2022. Crossref

  13. Bekeschus Sander, Schmidt Anke, Weltmann Klaus-Dieter, von Woedtke Thomas, The plasma jet kINPen – A powerful tool for wound healing, Clinical Plasma Medicine, 4, 1, 2016. Crossref

  14. Bekeschus Sander, Moritz Juliane, Schmidt Anke, Wende Kristian, Redox regulation of leukocyte-derived microparticle release and protein content in response to cold physical plasma-derived oxidants, Clinical Plasma Medicine, 7-8, 2017. Crossref

  15. Bekeschus Sander, Wende Kristian, Hefny Mohamed Mokhtar, Rödder Katrin, Jablonowski Helena, Schmidt Anke, Woedtke Thomas von, Weltmann Klaus-Dieter, Benedikt Jan, Oxygen atoms are critical in rendering THP-1 leukaemia cells susceptible to cold physical plasma-induced apoptosis, Scientific Reports, 7, 1, 2017. Crossref

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