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等离子医学

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ISSN 打印: 1947-5764

ISSN 在线: 1947-5772

SJR: 0.216 SNIP: 0.263 CiteScore™:: 1.4 H-Index: 24

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Nonthermal Atmospheric Pressure Plasma Decontamination of Protein-Loaded Biodegradable Nanoparticles for Nervous Tissue Repair

卷 1, 册 3-4, 2011, pp. 215-230
DOI: 10.1615/PlasmaMed.2012003626
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摘要

When injected directly into spinal tissue, poly(lactide-co-glycolide) (PLGA) particles have the potential to provide sustained delivery of proteins necessary to promote nerve regeneration. To ensure the injection of sterile particles, a novel decontamination method was developed that can inactivate bacteria while maintaining particle and protein integrity. This method involves the resuspension of protein-loaded particles after preparation in phosphate-buffered saline (PBS) or deionized water that has been subjected to a nonthermal atmospheric pressure discharge. Although different treatment times were required, it was demonstrated that both plasma-treated PBS and water can completely inactivate Escherichia coli when added at a concentration of 103 colony-forming units/mL to PLGA particles with and without protein. Plasma-treated water showed no impact on particle morphology. Plasma treatment of water for 1 minute resulted in the complete sterilization of protein-loaded particles but was able to maintain only 26.6 ± 5.0% of protein activity. On the other hand, plasma-treated PBS required 2 minutes of treatment for complete sterilization of protein-loaded particles but was able to maintain 68.4 ± 10.6% of protein activity. Particles were considered sterile if no bacterial growth was observed after being plated onto bacterial growth agar. The effect of plasma-treated water on release of active protein from particles caused a substantial loss in the initial burst release of protein but did not limit the ability of the particles to provide a sustained release of active protein.

对本文的引用
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  2. Kaushik Nagendra, Kaushik Neha, Linh Nguyen, Ghimire Bhagirath, Pengkit Anchalee, Sornsakdanuphap Jirapong, Lee Su-Jae, Choi Eun, Plasma and Nanomaterials: Fabrication and Biomedical Applications, Nanomaterials, 9, 1, 2019. Crossref

  3. Melo Tatiane Fonseca de, Rocha Lucas Cabral, Silva Rútilo Pereira, Pessoa Rodrigo Sávio, Negreiros Andreia Mitsa Paiva, Sales Júnior Rui, Tavares Moisés Bento, Alves Junior Clodomiro, Plasma–Saline Water Interaction: A Systematic Review, Materials, 15, 14, 2022. Crossref

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