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等离子医学
SJR: 0.278 SNIP: 0.183 CiteScore™: 0.57

ISSN 打印: 1947-5764
ISSN 在线: 1947-5772

等离子医学

DOI: 10.1615/PlasmaMed.2016015740
pages 125-143

Slow Molecular Transport of Plasma-Generated Reactive Oxygen and Nitrogen Species and O2 through Agarose as a Surrogate for Tissue

Jun-Seok Oh
Department of Electronic and Photonic Systems Engineering, Kochi University of Technology, 185 Miyanokuchi, Tosayamada-cho, Kami-shi, Kochi 782-8502, Japan; Center for Nanotechnology, Research Institute of Kochi University of Technology, 185 Miyanokuchi, Tosayamada-cho, Kami-shi, Kochi 782-8502, Japan
Endre J. Szili
Future Industries Institute, University of South Australia, Adelaide, South Australia 5095, Australia; Wound Management Innovation Cooperative Research Centre, Australia
Satsuki Ito
Department of Electronic and Photonic Systems Engineering, Kochi University of Technology, 185 Miyanokuchi, Tosayamada-cho, Kami-shi, Kochi 782-8502, Japan
Sung-Ha Hong
Future Industries Institute, University of South Australia, Adelaide, South Australia 5095, Australia; Wound Management Innovation Cooperative Research Centre, Australia
Nishtha Gaur
Future Industries Institute, University of South Australia, Adelaide, South Australia 5095, Australia; Wound Management Innovation Cooperative Research Centre, Australia
Hiroshi Furuta
Department of Electronic and Photonic Systems Engineering, Kochi University of Technology, 185 Miyanokuchi, Tosayamada-cho, Kami-shi, Kochi 782-8502, Japan; Center for Nanotechnology, Research Institute of Kochi University of Technology, 185 Miyanokuchi, Tosayamada-cho, Kami-shi, Kochi 782-8502, Japan
Robert D. Short
Future Industries Institute, University of South Australia, Adelaide, South Australia 5095, Australia; Wound Management Innovation Cooperative Research Centre, Australia
Akimitsu Hatta
Department of Electronic and Photonic Systems Engineering, Kochi University of Technology, 185 Miyanokuchi, Tosayamada-cho, Kami-shi, Kochi 782-8502, Japan; Center for Nanotechnology, Research Institute of Kochi University of Technology, 185 Miyanokuchi, Tosayamada-cho, Kami-shi, Kochi 782-8502, Japan

ABSTRACT

The helium (He) atmospheric-pressure plasma jet (APPJ) delivery of reactive oxygen and nitrogen species (RONS) and molecular oxygen (O2) in deionized (DI) water was monitored in real time using in situ UV absorption spectroscopy. The He APPJ was used to treat DI water directly and through an agarose target as a surrogate for tissue (e.g., a skin barrier). For direct treatment, the RONS were generated immediately in the DI water, and the concentration of RONS continued to increase during the He APPJ treatment. But there was only a very minor increase in the total RONS concentration detected after the plasma and gas flow were switched off. The agarose target delayed the generation of RONS into the DI water, but the total RONS concentration continued to increase long after (25 min) the plasma and gas flow were switched off. Direct treatment deoxygenated the DI water, whereas treatment through agarose resulted in oxygenation of the DI water. A dynamic change in the ratio of H2O2, NO2-, NO3-, and O2 was detected in the DI water during He APPJ treatment and 25 min after the He and gas flow were switched off for both direct and through-agarose treatment. These results have implications for the plasma treatment of real tissue where the dynamic changes in the RONS and O2 concentrations within the tissue and tissue fluid could affect cellular and physiological processes.