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环境病理学,毒理学和肿瘤学期刊
影响因子: 1.625 5年影响因子: 1.63 SJR: 0.402 SNIP: 0.613 CiteScore™: 2.3

ISSN 打印: 0731-8898
ISSN 在线: 2162-6537

环境病理学,毒理学和肿瘤学期刊

DOI: 10.1615/JEnvironPatholToxicolOncol.2020031451
pages 51-60

Evaluation of Cytotoxicity of Different Part Extracts of Ipomoea turpethum against Breast Cancer Cell Lines

Mohd Mughees
Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi-110062, India
Saima Wajid
Department of Biotechnology, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi 110062, India

ABSTRACT

The aim of the present study was to explore the anti-breast cancer activity of the plant I. turpethum together with the comparative analysis of the cytotoxicity of the extracts from different parts of the plant. The gas chromatography-mass spectrometry (GC-MS) analysis of the plant revealed the identification of 71 compounds. The cytotoxicity of the extracts from different parts (whole, aerial, and root) of the plant was evaluated against the breast cancer cell lines (MCF-7 and MDA MB-231) by 3-(4,5-dimethylthiazol-2-Yl)-2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. The findings of these assays were further confirmed by the carboxyfluorescein diacetate succinimidyl ester (CFSE) proliferation assay, DAPI (4',6-diamidino-2-phenylindole) nuclear staining, and simple microscopy images. The results of the MTT assay revealed that the root extract showed the lowest IC50 values, i.e., 516 μg/mL for MCF-7 and 396 μg/mL for MDA MB-231 cell lines. The LDH release in the medium was high in the root extract treated cells, i.e., 28.87% for MCF-7 and 23.62% for MDA MB-231 cell lines. Moreover, the CFSE proliferation assay also showed the decrease in the proliferation of the cells up to 48.7% and 46.4% in case of MCF-7 and MDA MB-231 cells, respectively. The fragmented and condensed nuclear material in both the cell lines was observed in the DAPI nuclear staining. In conclusion, results showed that the root extract possess the highest cytotoxicity among all other tested extracts toward both breast cancer cell lines (MCF-7 and MDA MB-231).

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