每年出版 4 期
ISSN 打印: 0731-8898
ISSN 在线: 2162-6537
Indexed in
A Simple One-Step Protocol for Preparing Small-Sized Doxorubicin-Loaded Liposomes
摘要
A simple, single-step, extrusion-free protocol for preparing doxorubicin-loaded liposomes (100150 nm), based on the ethanol injection method (EIM), is described. Efficient encapsulation of doxorubicin (up to 98%) was obtained concomitantly with liposome preparation avoiding the need for an additional loading step. Parameters such as stock concentration of phospholipid, injection ratio, lipid composition, and drug-to-phospholipid ratio affected the resultant liposome size and magnitude of doxorubicin encapsulation. A lipid stock concentration (50 mM) and injection ratio (1:10) resulted in 96.0 ± 2.92% encapsulation efficiency of doxorubicin (drug-to-lipid mole ratio: 0.192) and mean diameter of 135 ± 2.32 nm for SCOL-2 formulation (DSPC/ cholesterol /oleic acid: 2/2/1; molar ratios). Replacement of phospholipid DSPC with DMPC or DPPC did not affect the mean liposome size and doxorubicin-encapsulation efficiency. These findings offer promise for scale-up and development on a large-scale production of doxorubicin-loaded liposomes for effective cancer therapy.
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